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Chlorophyll is the green pigment which can be found ubiquitously in plants, algae, and bacteria. It is classified as a natural product that is needed in the photosynthesis. The degradation pathway and enzymes involved in chlorophyll degradation are known in plants during leaf senescence and fruit ripening. However, the enzymes involved in chlorophyll degradation in chewing insects are not known. We will use Spodoptera littoralis as the model organism. Thus, the complex of chlorophyll degradation will be characterized with several techniques such as LC-MS, NMR, and methods of Molecular Biology. A transcriptomic approach will be used to apprehend the genes and protein sequences. Expression of the sequences in bacteria or yeast can be used as a downstream process to produce and purify the protein(s). The chlorophyll degradation does not stop in the gut. It continues in the excrements under totally different conditions (presence of oxygen and light) and may result in further oxidative degradation of chlorophyll degradation products to yield ring-opened tetra¬pyrroles. We put some effort to characterize these compounds also. Eventually the combination of degradation process in the gut and excrements will provide complete information of novel pathways of chlorophyll degradation in insects.
last updated on 2019-01-16