Dr. Ruo Sun

   Department of Biochemistry
   Detox & Mode of Action
 Phone:+49 (0)3641 57 1319Max Planck Institute for Chemical Ecology
 Fax:+49 (0)3641 57 1302Hans-Knöll-Straße 8
  emailD-07745 Jena

 Research  Theses  Publications  Presentations  help    to staff list 

Glucosinolate (GLS)-myrosinase system is the characteristic defense system of Brassicaceae plants against herbivorous insects. GLSs and myrosinases are stored in different compartments of leaf tissue. Herbivore’s chewing ruptures these compartments, myrosinases come in contact with GLSs to catalyze their deglycosylation to produce toxic isothiocyanates (ITCs). A specialist herbivore Plutella xylostella has evolved a counter-adaptation glucosinolate sulfatase (GSS) which rapidly desulfatizes GLSs to form harmless desulfo-GLSs, before their deglycosylation to ITCs. To outcompete the myrosinases, GSS is present in high quantity in P. xylostella, indicating that its possession is resource-intensive. Secondly, it has been observed that P. xylostella larvae feeding on hosts of different glucosinolate compositions are differentially susceptible to their natural enemies. However, whether and how P. xylostella’s GSS influences the natural enemy success is unknown. We propose the use of a reverse genetic method, plant-mediated RNAi by which P. xylostella larvae’s GSSs will be silenced and the loss-of-function phenotypes will be studied in vivo for understanding the effect of GLSs desulfation on both herbivore and its natural enemy. In this study Diadegma semiclausum, a specialist hymenopteran endoparasitoid will be used as its natural enemy. This work will determine the physiological and ecological cost-benefit economics of the GSS mechanism.
last updated on 2017-08-04