Research Topic
Cytochrome P450 monooxygenases and insecticide resistance
Worldwide, increasing numbers of insects have evolved resistance to a wide range of pesticides, which hampers their control in the field and, therefore, threatens agriculture. The main mechanisms underlying insecticide resistance are target site insensitivity and metabolism mainly due to carboxylesterases and cytochrome P450 monooxygenases.
I am very interested in the metabolic activity of P450 enzymes and especially in their involvement in insecticide resistance. My research focuses on the cotton bollworm, Helicoverpa armigera, a major pest and the noctuid species possessing by far the most reported cases of insecticide resistance worldwide, and its resistance against pyrethroid insecticides, mainly fenvalertate. I work on the elucidation of the underlying genetic mechanism of the resistance observed, the characterization of the P450 genes and enzymes involved including their enzymatic capacities after heterologous expression in insect cell cultures and on the consequences for H. armigera itself.
Publications
2013
Bomble, F.W., Joußen, N., Wolgarten, H. (2013). Peltigera didactyla (Kleine Hundsflechte), P. praetextata (Schuppige Hundsflechte) und P. rufescens (Bereifte Hundsflechte) in Nordrhein-Westfalen. Pflanzenporträts 2013, BOCHUMER BOT. VER. (http://www.botanik-bochum.de/html/jahrbuch/2013/Pflanzenportraet_Peltigera.pdf)
2012
Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. PROC. NATL. ACAD. SCI. USA 109 (38), 15206-15211
2011
Bomble, F.W., Joußen, N., Wolgarten, H. (2011). Bemerkenswerte und ehemals seltenere Großflechten im Aachener Stadtgebiet und der nordwestlichen Eifel (Remarkable macrolichens in the urban area of Aachen and the north-western Eifel Mountains). ONLINE-VERÖFF. BOCHUMER BOT. VER. 3 (9), 109-126
Schmitz, B.G.A., Bomble, F.W., Joußen, N., Wolgarten, H. (2011). Ein Wiederfund von Parmelina quercina (Willd.) Hale im Rheinland und in Nordrhein-Westfalen (A rediscovery of Parmelina quercina (Willd.) Hale in the Rhineland and in North Rhine-Westphalia). DECHENIANA 164, 65-67
Bomble, F.W., Joußen, N., Schmitz, B.G.A., Wolgarten, H. (2011). Seltene und kritische Gefäßpflanzen im Nationalpark Eifel (Rare and critical vascular plants in the Eifel National Park). DECHENIANA 164, 47-56
2010
Joußen, N., Schuphan, I., Schmidt, B. (2010). Metabolism of methoxychlor by the P450-monooxygenase CYP6G1 involved in insecticide resistance of Drosophila melanogaster after expression in cell cultures of Nicotiana tabacum. CHEM. BIODIV. 7, 722-735
2008
Joußen, N. (2008). Untersuchungen zur Metabolismus-bedingten Insektizid-Resistenz von Drosophila melanogaster als Beispiel für den Einsatz Cytochrom P450-transgener Zellsuspensionskulturen von Nicotiana tabacum zur Aufklärung der enzymatischen Kapazität des Ursprungsorganismus. Dissertation RWTH Aachen, VERLAG DR. HUT, ISBN 978-3-89963-746-5
for online publication see: http://darwin.bth.rwth-aachen.de/opus3/volltexte/2008/2401/
Joußen, N., Heckel, D.G., Haas, M., Schuphan, I., Schmidt, B. (2008). Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1-overexpressing strains of Drosophila melanogaster, leading to resistance. PEST MANAG. SCI. 64, 65-73
2006
Schmidt, B., Joussen, N., Bode, M., Schuphan, I. (2006). Oxidative metabolic profiling of xenobiotics by human P450s expressed in tobacco cell suspension cultures. BIOCHEM. SOC. TRANS. 34, 1241-1245
Schmidt, B., Faymonville, T., Gembé, E., Joußen, N., Schuphan, I. (2006). Comparison of the biotransformation of the 14C-labelled insecticide carbaryl by non-transformed and human CYP1A1-, CYP1A2-, and CYP3A4-transgenic cell cultures of Nicotiana tabacum, CHEM. BIODIV. 3, 878-896
Data base entries
CYP337B1v1, v3, v4, CYP337B2v1, v2, v3 & CYP337B3
Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. PROC. NATL. ACAD. SCI. USA 109 (38), 15206-15211, NCBI data base (and associated data bases), accession numbers: JQ284023-JQ284029
CYP71U4v2
Joussen, N., Faymonville, T., Schuphan, I., Schmidt, B. (2005). Identification of cytochrome P450 monooxygenase from barley, NCBI data base (and associated data bases), accession number: AY989887
Talk presentations
Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). The chimeric P450 enzyme CYP337B3 is responsible for fenvalerate resistance of Australian Helicoverpa armigera, 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Torino, IT
Joußen, N., Schmidt, B., Heckel, D.G. (2010). Gene duplication and functional diversification in insect cytochromes P450, ICE Symposium, MPI for Chemical Ecology, Jena, DE
Joußen, N., Schuphan, I., Schmidt, B. (2004) Influence of a recombinant human P450 system on endogenous ingredients in transgenic plants of Nicotiana tabacum L., 24. Tagung des Arbeitskreises Phytopharmakologie der Deutschen Phytomedizinischen Gesellschaft e.V. in Zusammenarbeit mit SYNGENTA, Stein/Aargau, CH
Poster Presentations
Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). The chimeric P450 enzyme CYP337B3 is responsible for fenvalerate resistance of Australian Helicoverpa armigera, 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Torino, IT
Joußen, N., Schmidt, B., Heckel, D.G.(2010). The role of a new chimeric cytochrome P450-monooxygenase of the Cyp337b cluster in fenvalerate resistance of Australian Helicoverpa armigera, SAB Meeting 2010, MPI for Chemical Ecology, Jena, DE
Joußen, N., Schmidt, B., Heckel, D.G. (2010). Does one of the cytochrome P450-monooxygenases of the Cyp337b cluster cause the fenvalerate resistance in Australian Helicoverpa armigera?, 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Woods Hole, MA, US
Joußen, N., Schmidt, B., Heckel, D.G. (2009). Does the cytochrome P450-monooxygenase CYP337B1 play a role in the insecticide resistance of Helicoverpa armigera?, ICE Symposium, MPI for Chemical Ecology, Jena, DE
Joußen, N., Heckel, D.G., Haas, M., Schuphan, I., Schmidt, B. (2008). Metabolism of imidacloprid, DDT and methoxychlor by CYP6G1 expressed in tobacco cell suspension cultures with regard to insecticide resistance of Drosophila melanogaster, 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Nice, FR
Joußen, N., Berger, A., Schuphan, I., Schmidt, B. (2006). Metabolism of imidacloprid and DDT in tobacco cell suspension cultures expressing the P450 isozyme CYP6G1 from insecticide-resistant Drosophila melanogaster, 11th IUPAC International Congress of Pesticide Chemistry, Kobe, JP
Schmidt, B., Joussen, N., Bode, M., Faymonville, F., Stöbe, P., Breuer, M., Berger, A., Gasthaus, M., Schuphan, I.(2006). Oxidative metabolic profiling of pesticides using transgenic tobacco cell suspension cultures, which express human P450 isozymes, 11th IUPAC International Congress of Pesticide Chemistry, Kobe, JP
Joußen, N., Schmidt, B., Schuphan, I. (2006). Relevance of cytochrome P450 6G1 in insecticide resistance, 8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Swansea, Wales, UK
Awards
2009
“Borchers-Plakette” by the RWTH Aachen University
2004
“Springorum-Denkmünze” by the RWTH Aachen University |
