nijo4362

Dr. Nicole Joußen

   
   Department of Entomology
 Phone:+49 (0)3641 57 1552Max Planck Institute for Chemical Ecology
 Fax:+49 (0)3641 57 1502Hans-Knöll-Straße 8
  emailD-07745 Jena

  Research    CV   Publications   Presentations   IMPRS   Awards    to staff list 

Research Topic



Cytochrome P450 monooxygenases and insecticide resistance

Worldwide, increasing numbers of insects have evolved resistance to a wide range of pesticides, which hampers their control in the field and, therefore, threatens agriculture. The main mechanisms underlying insecticide resistance are target site insensitivity and metabolism mainly due to carboxylesterases and cytochrome P450 monooxygenases.
I am very interested in the metabolic activity of P450 enzymes and especially in their involvement in insecticide resistance. My research focuses on the cotton bollworm, Helicoverpa armigera, a major pest and the noctuid species possessing by far the most reported cases of insecticide resistance worldwide, and its resistance against pyrethroid insecticides, mainly fenvalerate. I work on the elucidation of the underlying genetic mechanism of the resistance observed, the characterization of the P450 genes and enzymes involved including their enzymatic capacities after heterologous expression in insect cell cultures and on the consequences for H. armigera itself.

Publications



2014

Rasool*, A., Joußen*, N., Lorenz, S., Ellinger, R., Schneider, B., Khan, S.A., Ashfaq, M., Heckel, D.G. (2014). An independent occurrence of the chimeric P450 enzyme CYP337B3 of Helicoverpa armigera confers cypermethrin resistance in Pakistan. INSECT BIOCHEM. MOL. BIOL. 53, 54-65 (* These authors contributed equally to this work.)

2013

Bomble, F.W., Joußen, N., Wolgarten, H. (2013). Peltigera didactyla (Kleine Hundsflechte), P. praetextata (Schuppige Hundsflechte) und P. rufescens (Bereifte Hundsflechte) in Nordrhein-Westfalen. Pflanzenporträts 2013, BOCHUMER BOT. VER. (http://www.botanik-bochum.de/html/jahrbuch/2013/ Pflanzenportraet_Peltigera.pdf)

2012

Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. PROC. NATL. ACAD. SCI. USA 109 (38), 15206-15211

2011

Bomble, F.W., Joußen, N., Wolgarten, H. (2011). Bemerkenswerte und ehemals seltenere Großflechten im Aachener Stadtgebiet und der nordwestlichen Eifel (Remarkable macrolichens in the urban area of Aachen and the north-western Eifel Mountains). ONLINE-VERÖFF. BOCHUMER BOT. VER. 3 (9), 109-126

Schmitz, B.G.A., Bomble, F.W., Joußen, N., Wolgarten, H. (2011). Ein Wiederfund von Parmelina quercina (Willd.) Hale im Rheinland und in Nordrhein-Westfalen (A rediscovery of Parmelina quercina (Willd.) Hale in the Rhineland and in North Rhine-Westphalia). DECHENIANA 164, 65-67

Bomble, F.W., Joußen, N., Schmitz, B.G.A., Wolgarten, H. (2011). Seltene und kritische Gefäßpflanzen im Nationalpark Eifel (Rare and critical vascular plants in the Eifel National Park). DECHENIANA 164, 47-56

2010

Joußen, N., Schuphan, I., Schmidt, B. (2010). Metabolism of methoxychlor by the P450-monooxygenase CYP6G1 involved in insecticide resistance of Drosophila melanogaster after expression in cell cultures of Nicotiana tabacum. CHEM. BIODIV. 7, 722-735

2008

Joußen, N. (2008). Untersuchungen zur Metabolismus-bedingten Insektizid-Resistenz von Drosophila melanogaster als Beispiel für den Einsatz Cytochrom P450-transgener Zellsuspensionskulturen von Nicotiana tabacum zur Aufklärung der enzymatischen Kapazität des Ursprungsorganismus. Dissertation RWTH Aachen, VERLAG DR. HUT, ISBN 978-3-89963-746-5
for online publication see: http://darwin.bth.rwth-aachen.de/opus3/volltexte/2008/2401/

Joußen, N., Heckel, D.G., Haas, M., Schuphan, I., Schmidt, B. (2008). Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1-overexpressing strains of Drosophila melanogaster, leading to resistance. PEST MANAG. SCI. 64, 65-73

2006

Schmidt, B., Joussen, N., Bode, M., Schuphan, I. (2006). Oxidative metabolic profiling of xenobiotics by human P450s expressed in tobacco cell suspension cultures. BIOCHEM. SOC. TRANS. 34, 1241-1245

Schmidt, B., Faymonville, T., Gembé, E., Joußen, N., Schuphan, I. (2006). Comparison of the biotransformation of the 14C-labelled insecticide carbaryl by non-transformed and human CYP1A1-, CYP1A2-, and CYP3A4-transgenic cell cultures of Nicotiana tabacum, CHEM. BIODIV. 3, 878-896

Data base entries



CYP337B3v2

Rasool*, A., Joußen*, N., Lorenz, S., Ellinger, R., Schneider, B., Khan, S.A., Ashfaq, M., Heckel, D.G. (2014). An independent occurrence of the chimeric P450 enzyme CYP337B3 of Helicoverpa armigera confers cypermethrin resistance in Pakistan. INSECT BIOCHEM. MOL. BIOL. 53, 54-65 (* These authors contributed equally to this work.), NCBI data base (and associated data bases), accession number: KJ636466

CYP337B1v1, v3, v4, CYP337B2v1, v2, v3 & CYP337B3

Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3. PROC. NATL. ACAD. SCI. USA 109 (38), 15206-15211, NCBI data base (and associated data bases), accession numbers: JQ284023-JQ284029

CYP71U4v2

Joussen, N., Faymonville, T., Schuphan, I., Schmidt, B. (2005). Identification of cytochrome P450 monooxygenase from barley, NCBI data base (and associated data bases), accession number: AY989887

Talk presentations



Joußen, N., Rasool, A., Pavlidi, N., Ahn, S.-J., Vontas, J., Heckel, D.G. (2014). Crucial amino acids and the evidence for multiple origin - The chimeric CYP337B3 of Helicoverpa armigera, 12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Kyoto, JP (invited)

Joußen, N., Rasool, A., Pavlidi, N., Ahn, S.-J., Vontas, J., Heckel, D.G. (2014). A worldwide resistance mechanism of Helicoverpa armigera - Crucial amino acids and the evidence for multiple origin in the chimeric CYP337B3, ICE Symposium, MPI for Chemical Ecology, Jena, DE

Joußen, N., Pavlidi, N., Rasool, A., Ahn, S.-J., Vontas, J., Heckel, D.G. (2014). A worldwide resistance mechanism of the insect pest Helicoverpa armigera (Lepidoptera: Noctuidae): the chimeric P450 enzyme CYP337B3, 10th European Congress of Entomology, Royal Entomological Society, York, UK (invited)

Joußen, N. (2014). Das juckt mich nicht! - Wie Insekten giftigen Insektiziden trotzen. Institute Seminar, MPI for Chemical Ecology, Jena, DE

Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). The chimeric P450 enzyme CYP337B3 is responsible for fenvalerate resistance of Australian Helicoverpa armigera, 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Torino, IT

Joußen, N., Schmidt, B., Heckel, D.G. (2010). Gene duplication and functional diversification in insect cytochromes P450, ICE Symposium, MPI for Chemical Ecology, Jena, DE

Joußen, N., Schuphan, I., Schmidt, B. (2004). Influence of a recombinant human P450 system on endogenous ingredients in transgenic plants of Nicotiana tabacum L., 24. Tagung des Arbeitskreises Phytopharmakologie der Deutschen Phytomedizinischen Gesellschaft e.V. in Zusammenarbeit mit SYNGENTA, Stein/Aargau, CH

Poster Presentations



Krempl, C., Joußen, N., Heidel-Fischer, H., Reichelt, M., Vogel, H., Heckel, D.G. (2014). Adaptation of generalist herbivores to gossypol, a toxic cotton secondary metabolite. ICE Symposium, MPI for Chemical Ecology, Jena, DE

Joußen, N., Pavlidi, N., Rasool, A., Ahn, S.J., Vontas, J., Heckel, D.G. (2014). Universal weapon CYP337B3: Pyrethroid resistance of Helicoverpa armigera. SAB Meeting 2014, MPI for Chemical Ecology, Jena, DE

Krempl, C., Joußen, N., Heidel-Fischer, H., Vogel, H., Heckel, D.G. (2014). Detoxification of Gossypol, a cotton secondary metabolite, by Heliothine moth. SAB Meeting 2014, MPI for Chemical Ecology, Jena, DE

Joußen, N., Heckel, D.G. (2013) The amino acid puzzle - Crucial amino acids in the chimeric resistance enzyme CYP337B3 of Helicoverpa armigera. ICE Symposium, MPI for Chemical Ecology, Jena, DE

Krempl, C., Joußen, N., Heidel-Fischer, H., Heckel, D.G., Vogel, H. (2013) Detoxification of gossypol, a cotton secondary metabolite, by heliothine moths. ICE Symposium, MPI for Chemical Ecology, Jena, DE

Krempl, C., Joußen, N., Heidel-Fischer, H., Heckel, D.G., Vogel, H. (2013). Detoxification of Gossypol, a cotton secondary metabolite by Heliothine moths. International Chemical Ecology Conference 2013, International Society of Chemical Ecology, Asia-Pacific Association of Chemical Ecologists, Melbourne, AU

Joußen, N. (2012). Structure-function relationship of a chimeric P450 enzyme conferring insecticide resistance to Helicoverpa armigera and its parent enzymes. SAB Meeting 2012, MPI for Chemical Ecology, Jena, DE

Joußen, N., Agnolet, S., Lorenz, S., Schöne, S.E., Ellinger, R., Schneider, B., Heckel, D.G. (2012). The chimeric P450 enzyme CYP337B3 is responsible for fenvalerate resistance of Australian Helicoverpa armigera, 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Torino, IT

Joußen, N., Schmidt, B., Heckel, D.G. (2010). The role of a new chimeric cytochrome P450-monooxygenase of the Cyp337b cluster in fenvalerate resistance of Australian Helicoverpa armigera, SAB Meeting 2010, MPI for Chemical Ecology, Jena, DE

Joußen, N., Schmidt, B., Heckel, D.G. (2010). Does one of the cytochrome P450-monooxygenases of the Cyp337b cluster cause the fenvalerate resistance in Australian Helicoverpa armigera?, 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Woods Hole, MA, US

Joußen, N., Schmidt, B., Heckel, D.G. (2009). Does the cytochrome P450-monooxygenase CYP337B1 play a role in the insecticide resistance of Helicoverpa armigera?, ICE Symposium, MPI for Chemical Ecology, Jena, DE

Joußen, N., Heckel, D.G., Haas, M., Schuphan, I., Schmidt, B. (2008). Metabolism of imidacloprid, DDT and methoxychlor by CYP6G1 expressed in tobacco cell suspension cultures with regard to insecticide resistance of Drosophila melanogaster, 9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Nice, FR

Joußen, N., Berger, A., Schuphan, I., Schmidt, B. (2006). Metabolism of imidacloprid and DDT in tobacco cell suspension cultures expressing the P450 isozyme CYP6G1 from insecticide-resistant Drosophila melanogaster, 11th IUPAC International Congress of Pesticide Chemistry, Kobe, JP

Schmidt, B., Joussen, N., Bode, M., Faymonville, F., Stöbe, P., Breuer, M., Berger, A., Gasthaus, M., Schuphan, I.(2006). Oxidative metabolic profiling of pesticides using transgenic tobacco cell suspension cultures, which express human P450 isozymes, 11th IUPAC International Congress of Pesticide Chemistry, Kobe, JP

Joußen, N., Schmidt, B., Schuphan, I. (2006). Relevance of cytochrome P450 6G1 in insecticide resistance, 8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Swansea, Wales, UK

Awards



“Borchers-Plakette” by the RWTH Aachen University (2009)

“Springorum-Denkmünze” by the RWTH Aachen University (2004)

Journal Referee (Reviewer)



Insect Biochemistry and Molecular Biology; Journal of Applied Entomology; PLoS ONE; Zeitschrift für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie; International Journal of Molecular Sciences; Pesticide Biochemistry and Physiology; Journal of Agricultural Science; Biological Research; Phytoparasitica

Advanced training and teaching



Unbehend, M., Beran, F., Hänniger, S., Joußen, N., Kuwar, S. (SS2014). Geländepraktikum Ökologie: Sexuelle Kommunikation und Schädlingsbekämpfung von Insekten, Joußen, N. "Resistenzmechanismen bei Insekten" (Presentation) and "Schädlingsbekämpfung mit Insektiziden" (Practical training), FSU/MPI for Chemical Ecology, Jena, DE

Joußen, N. (2014). Resistenzmechanismen bei Insekten, 20. Tage des mathematischen und naturwissenschaftlichen Unterrichts (Lehrerfortbildung), Erfurt, Germany, DE

Unbehend, M., Joußen, N. (SS2013). Geländepraktikum Ökologie: Sexuelle Kommunikation und Schädlingsbekämpfung von Nachtfaltern, Joußen, N. "Insektizidresistenz durch Neukombination zweier Gene – Das chimäre CYP337B3 der Baumwolleule" (Presentation) and "Schädlingsbekämpfung mit Insektiziden" (Practical training), FSU/MPI for Chemical Ecology, Jena, DE

Activities



"Das große Krabbeln – Was macht Insekten so interessant?" (2013) and "Tiere, Pflanzen, Mikroben: Große Ausstellung lebendiger Forschungsobjekte" (2013), Lange Nacht der Wissenschaften 2013, MPI for Chemical Ecology, Jena, DE

Joußen, N., Heckel, D.G. (2013). Pyrethroid-resistente Raupen: Insektizid-Resistenz durch Neukombination zweier Gene (Talk Presentation), Sitzung des Kuratoriums 2013, MPI for Chemical Ecology, Jena, DE

Joußen, N., Krempl, C. (2012). "Biokatalysatoren" – Die Rolle von Enzymen bei der Insektizidresistenz von Insekten, Forsche Schüler Tag 2012, MPI for Chemical Ecology, Jena, DE

Es war einmal … Der Darm – Die Bakterien-Gemeinschaft im Darm von Raupen (2011), Lange Nacht der Wissenschaften 2011, MPI for Chemical Ecology, Jena, DE

Joußen, N. (2011). "Biokatalysatoren" – Die Rolle von Enzymen bei der Insektizidresistenz von Insekten, Forsche Schüler Tag 2011, MPI for Chemical Ecology, Jena, DE

Joußen, N. (2010). Faszination Pflanze, jun.iversity-Sommercamp 2010

Joußen, N. (2010). "Biokatalysatoren" – Die Rolle von Enzymen bei der Insektizidresistenz von Insekten, Forsche Schüler Tag 2010, MPI for Chemical Ecology, Jena, DE

Die Raupe, die Deine Kleidung raubt: Du, die Baumwolle und ein Baumwollschädling (2009), Lange Nacht der Wissenschaften 2009, MPI for Chemical Ecology, Jena, DE

Qualifications



MS Excel training, in-house course, MPI for Chemical Ecology, Jena, DE, May-Jun 2013

Statistic R course, in-house course, MPI for Chemical Ecology, Jena, DE, Jan-Feb 2013

Interactive NMR exercise course, IMPRS in-house course, MPI for Chemical Ecology, Jena, DE, Mar-Apr 2012

Qualitätssicherung, Standardisierung, Troubleshooting und Kontamination in der Zellkultur, Inhouse training Beutenberg-Campus, Jena, DE, Nov 2008

HPLC-Methodenentwicklung mit Phenomenex® Phasen, in-house course, MPI for Chemical Ecology, Jena, DE, Sep 2008


last updated on 2014-10-06