dane4566

David Neunemann

   Department of Entomology
   Host Plant Adaptation
 Phone:+49 (0)3641 57 1560Max Planck Institute for Chemical Ecology
 Fax:+49 (0)3641 57 1502Hans-Knöll-Straße 8
  emailD-07745 Jena

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PhD Thesis

started in Nov 2011
RNA interference and insect-virus interactions
Friedrich-Schiller-Universität Jena, Biologisch-Pharmazeutische Fakultät
First Supervisor: Prof. D.G. Heckel
Co-Supervisor(s): Dr. H. Vogel, Dr. M. Schmidtke (FSU Jena, Virologie)

Diploma Thesis

started in Oct 2010
Silencing candidate genes (fatty acyl reductase and PBAN-Receptor) in the pheromone biosynthetic pathway
Friedrich-Schiller-Universität Jena, Biologisch-Pharmazeutische Fakultät
First Supervisor: Prof. D.G. Heckel
Co-Supervisor(s): Dr. A.T. Groot

Identification of the RNAi core machinery in Lepidoptera species


It was shown, that RNAi in Lepidopteran is not as straight-forward as for other insects and it has been proven to be difficult to establish. There have been several studies done in the past years with RNAi in different moth species which were sometimes successful and sometimes not (1). An important role in that random success could be the tissue in which a gene should be silenced, and also how the dsRNA is delivered to that tissue. To address these questions I want to identify the genes which are involved in the RNAi pathways (miRNA-pathway and siRNA-pathway) and in the transport or spreading of dsRNA in Helicoverpa armigera, Heliothis virescens and Spodoptera frugiperda and analyse the expression level of these RNAi core machinery genes in different tissues.

(1) Terenius et al., 2011 - RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
last updated on 2014-09-25